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Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid, 5(or 6)-carboxy-3',6'-dihydroxy-3-oxo- (85138-49-4)

Identification
Name:Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid, 5(or 6)-carboxy-3',6'-dihydroxy-3-oxo-
Synonyms:2',7'-Bis(carboxyethyl)-5(6)-carboxyfluorescein;5(or 6)-Carboxy-3',6'-dihydroxy-3-oxo-spiro[benzofuran-2(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid; 9-[2,4(or2,5)-Dicarboxyphenyl]-6-hydroxy-3-oxo-3H-xanthene-2,7-dipropanoic acid; BCECF;BCECF acid
CAS:85138-49-4
Molecular Formula: C27H20 O11
Molecular Weight: 250.27216
InChI: InChI=1S/C8H14N2O5S/c9-4(7(12)13)1-2-6(11)10-5(3-16)8(14)15/h4-5,16H,1-3,9H2,(H,10,11)(H,12,13)(H,14,15)/t4-,5-/m0/s1
Molecular Structure: (C27H20O11) 2',7'-Bis(carboxyethyl)-5(6)-carboxyfluorescein;5(or 6)-Carboxy-3',6'-dihydroxy-3-oxo-spiro[benzofur...
Properties
Flash Point: 318.2°C
Boiling Point: 936.2°Cat760mmHg
Density:1.67g/cm3
Refractive index:1.743
Water Solubility:2 mg/ml acetonitrile, 0.8 mg/ml DMSO
Solubility:2 mg/ml acetonitrile, 0.8 mg/ml DMSO
Appearance:reddish-brown or red crystalline powder
Specification:

 BCECF ,its CAS NO. is 85138-49-4,the synonyms is 2',7'-Bis(carboxyethyl)-5(6)-carboxyfluorescein ; Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid, ar-carboxy-3',6'-dihydroxy-3-oxo-, (2S-(1(R*(R*)),2alpha,3abeta,7abeta))- ; Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid,5(or 6)-carboxy-3',6'-dihydroxy-3-oxo- .

Flash Point: 318.2°C
Storage Temperature: −20°C
Usage:This fluorescent indicator is useful for pH measurements in intercellular spaces of epithelial cell monolayers, interstitial spaces of normal and neoplastic tissue and isolated cell fractions. Reliable measure of in vitro cellular cytotoxicity.
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